ABSTRACT
Background and purpose:The previous research has found that the prostate stromal cells derived from different prostate zones have distinct effect on prostate epithelial cells. We also revealed that LMO2 protein was highly expressed in PZ stromal cells (PZSCs) and prostate cancer associated fibroblasts (CAFs) compared with TZ stromal cells. This study investigated the effect of LMO2 protein in prostate stromal cells on proliferation and invasion of prostate cancer PC-3 cells and its mechanisms. Methods:Lentivirus overexpression vectors were used to establish LMO2-overexpressed prostate WPMY-1 stromal cell line. shRNA plasmids were used to suppress LMO2 in CAFs. LMO2 mRNA and protein level of both WPMY-1 and CAFs were evaluated by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. Then, PC-3 cells were co-cultured with different prostate stromal cells and the in vitro proliferation and invasion of PC-3 were measured by CCK-8 and matrigel invasion assays respectively. Results:When co-cultured with LMO2-overexpressed prostate stromal cells, both proliferation and in-vasion of PC-3 were improved. However, when co-cultured with CAFs which have inhibited expression of LMO2, the proliferation and invasion of PC-3 were reduced. The protein array proifling found that both interleukin-11 (IL-11) and ifbroblast growth factor-9 (FGF-9) were enhanced extensively in the supernatant collected from LMO2-overexpressed WPMY-1 cells. Conclusion:The expression of LMO2 in prostate stromal cells could be responsible for development of prostate cancer. Paracrine of cytokines, such as IL-11 and FGF-9, from LMO2-overexpressed stromal cells had effects on the proliferation and invasion of prostate cancer cells.
ABSTRACT
Objective To investigate the effect of LMO2 gene,which is overexpressed in prostate peripheral zone than transitional zone,on proliferation and invasion of prostate cancer PC-3 cell line or normal prostate epithelial BPH-1 cell line.Methods Lentivirus overexpression system was used to establish the LMO2 protein overexpressed prostate WPMY-1 stromal cell line.The LMO2 mRNA and protein expression level of those cells was evaluated by qRT-PCR and western blot.The PC-3 or BPH-1 cells was cocultured with prostate stromal cells and the in vitro proliferation and invasion activity were detected by Cell Counting Kit-8 (CCK-8),EdU and Matrigel invasive assays.Results The stable LMO2 overexpressed prostate stromal cells WPMY-1-LMO2 was successfully established.The results of CCK-8,EdU experiments indicated the proliferation and invasion activity of PC-3 or BPH-1 cells were both enhanced through cocultured with WPMY-1-LMO2 cells.The proportion of EdU positive cells of PC-3 cultured in WPMY-1-LMO2 supernatant was (42.67 ± 6.03) ％,and the difference was significant compared with the control group (29.33 ± 3.51) ％ (P ＜ 0.05).The BPH-1 culturcd in the supernatant was (35.00 ± 2.52) ％,aud the difference was significant compared with the control group (23.33 ± 2.52) ％ (P ＜ 0.05).The number of invaded PC-3 or BPH-1 cells after co-cultured with WPMY-1-LMO2 cells was 38 ± 5 and 43 ± 3,respectively,which was significantly different compared with the control group (P ＜ 0.05).Conclusions Tbe LMO2 overexpressed prostate stromal cells could induce proliferation and invasion of PC-3 or BPH-1 cells.The propensity of benign prostatic hyperplasia and prostate cancer at different zones may probably be related to distinctive gene expression between peripheral zone and transitional zone derived stromal cells.